FastQCFastQC Report
Thu 12 May 2022
EGAF00005408082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31620610
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT682330.2157864759724749No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT577470.18262456037375624TruSeq Adapter, Index 4 (97% over 38bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG559700.1770048079401378No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC556530.17600229723588506No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG496740.15709374360583175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC468460.148150209625937Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC450460.14245771982260938No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA423080.133798810332881No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA420750.13306194915278358No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA401960.12711962229697654No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC394220.12467185168154567Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT364160.11516539370998852No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC361260.11424827035278573No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC347060.10975752839682727Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC346920.10971325347613473Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT339490.10736352018509447Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG338120.10693025846117453No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA328020.10373613918264069Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)