FastQCFastQC Report
Wed 8 May 2024
EGAF00005408084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4137310
Total Bases624.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT98840.238899188119817TruSeq Adapter, Index 4 (97% over 38bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT88360.2135687197720258No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG74560.18021371374153736Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG74500.1800686919761874No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC71440.17267258194334Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC70960.1715124078205404No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC58950.14248388445632548No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC58680.14183128651225071Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA57860.1398493223858014No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA54460.13163142234930425No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA51840.12529880526235646No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC51640.12481539937785663Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC50530.12213249671888256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT49500.11964295641370842Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT49430.11947376435413348No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA48010.11604158257418468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC48000.11601741227995968Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTAT46850.11323782844408566TruSeq Adapter, Index 4 (97% over 38bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC46600.11263357108846085No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG45780.11065160696201155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG45070.10893551607203715No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG41990.10149106545073973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)