FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19104663
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT543020.2842342730672611TruSeq Adapter, Index 7 (97% over 35bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC481380.2519698986577256No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG422240.22101410529984225No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT338800.17733890411989994No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT310470.16251006364257772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284460.14889558638118872No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTAT260120.13615524126230336TruSeq Adapter, Index 7 (97% over 35bp)
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA232060.12146772753855956No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT230400.12059882972026255No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT224830.11768331113718154No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC214950.11251179882105222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)