FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70709506
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1858790.2628769602774484No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA1839200.26010646998438935No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1543560.21829596716458466No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA1491430.210923549656817No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT1238560.17516173850797373No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA1219530.17247044548720225No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1161540.16426928509442562No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1129120.15968432872377866No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC1098670.15537797704314327No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1036350.14656445202714327No Hit
CTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCC1006180.14229769898265163No Hit
CTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACA948960.13420543483927042No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC941830.1331970838546093No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA916970.12968129065984424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT905470.12805491810393924TruSeq Adapter, Index 22 (97% over 37bp)
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC863510.12212077963039368No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC846260.11968122079653618Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCA834730.11805060552961578No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC811300.11473704822658498No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT806760.1140949846262538No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG789550.11166108274041683No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA788420.11150127395883659No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG762350.10781435808645021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)