FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70709506
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT1967790.2782921436334176No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1867350.26408754715384375No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC1083580.15324389340239486No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG915660.12949602561217158No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG831170.1175471371557878No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA822710.11635069264944377No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC817090.11555589145255801Illumina Single End PCR Primer 1 (96% over 33bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGG813880.11510192137390975No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT788200.11147016074472363No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT725110.10254773947932828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)