FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70704563
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT2074670.293428021045827No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1657020.2343582832129236Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1533300.21686011976341613Illumina Single End PCR Primer 1 (100% over 34bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1353890.19148551982422973No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1309610.18522284056829544No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1223750.1730793527427643No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA1162680.16444200355216113No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC956630.13529961284111183No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG921860.13038196700261057No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC872490.12339939078613639No Hit
CACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGT850230.12025107912766535No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA831500.11760202803318366No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA824150.11656249116482058No Hit
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGG823860.11652147542443618No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT792080.11202671601265676No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG774700.10956860026134381No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG747500.10572160668046275No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA717350.10145738401636116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)