FastQCFastQC Report
Thu 12 May 2022
EGAF00005408114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33285734
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC894430.2687127163847431Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC809030.2430560792200046Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC776830.2333822652070704Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG715560.21497497997190027Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC557630.1675282269575308Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT549860.16519389357614886No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA496050.1490278087303107TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC483950.14539261775029505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT427310.1283763188157425No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT339640.10203770780599279Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)