FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25038446
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC744630.2973946546043632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC669450.26736882951921215Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC646610.25824685765242783Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG595560.2378582121270625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC464960.18569842553327792Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT425920.17010640356833645No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA415310.16586892013985213TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC398710.1592391157182838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT323900.1293610633822882No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT281210.11231128321621878Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG274860.10977518333206461TruSeq Adapter, Index 6 (97% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG260200.103920187379041Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)