FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25038446
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT689880.2755282815874436Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG616050.2460416273438056Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG595560.2378582121270625Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG540780.215979857535887Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT428750.1712366654064713Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCA387030.15457428947467428Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG358320.14310792291182928Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAAC254380.10159576197340682Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)