FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8529306
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT117060.13724446045199926No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG115630.13556788793836216Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC107620.12617673700533197Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT102500.12017390394951243No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC94110.11033723025062063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC92100.10798064930487897Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC90540.10615166110818394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)