FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50039501
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG777140.15530530570238898Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC717490.14338472320097675Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT712680.14242348260027612No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC615990.12310074794710682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT604230.12075060460734811No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC603240.1205527609078276Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC598690.11964347925851619Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)