FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22714797
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT463670.20412685176099088No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG337770.14870042642247694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT329860.14521811487023195TruSeq Adapter, Index 6 (97% over 37bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA314550.13847801501373752No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG301510.13273726373165476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC276580.12176203907963605Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA244950.10783719528728343No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC239650.10550391447478047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC227500.1001549782725331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)