FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22714797
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG339800.14959411699783184No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG303120.13344605280866037Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG280490.12348338398093543Illumina Single End PCR Primer 1 (100% over 32bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT252140.11100253284235821No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC243710.10729129562549028No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG236570.10414797015355233Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)