FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261538
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT168770.20428399651493462No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT147100.17805401367154639TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG123050.14894321130036564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG119350.14446462632018398No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA117190.1418501010344563No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC111810.13533799638759755Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC99070.1199171389152964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC84890.10275326458584345No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA84110.10180913045488625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)