FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005408130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65072586
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2390790.36740356376800515Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC2100260.32275649841240367Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTAT1999610.30728915552856617TruSeq Adapter, Index 1 (97% over 36bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1967070.3022885858570305Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA1939590.2980656093796549No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC1838820.2825798255505014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCCGAGATCTCGTAT1825010.2804575800937126TruSeq Adapter, Index 1 (97% over 36bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1747500.2685462661649869Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1339480.20584397859952885Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG1236180.18996939817329528No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1158460.1780258125902665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1108660.1703728202841055No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1019120.15661280158744575Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCCGAGATCGCGTAT1004380.15434763880445754TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC934500.14360886164874406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG865700.13303605300087504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTGGTAT838570.1288668626140046TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCGCGTAT833290.12805546102009838TruSeq Adapter, Index 1 (97% over 36bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG783510.12040554220482338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG755320.11607345680099451No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT751510.11548795678720991No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCCGAGATCTGGTAT743120.11419862736052935TruSeq Adapter, Index 1 (97% over 36bp)
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC722470.11102524802072565No Hit
CCCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACC668310.10270223470141482No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC665310.10224121106851354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG659490.10134682522068508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656430.10087658111512582No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA655100.10067219397120625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)