FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19779078
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG964970.48787410616409926Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC926000.46817146886219874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC902160.4561183286703253Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC771870.39024569294888267Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC758970.3837236498081458Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA708460.35818656461135345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG708380.3581461178321861Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT682260.34494024443404286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT617730.31231486118817064TruSeq Adapter, Index 18 (97% over 38bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC583940.29523115283735674Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC559630.28294038781787506No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT480510.2429385232213554TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG459860.23249819834878044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG421060.21288151045261056No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC368130.18612091018600563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA347800.17584237243010015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT338000.17088764198209846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT322290.16294490572310802No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA303350.1533691307552354No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG290080.14666002126084945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA275840.1394604945690593No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCA269050.13602757418722955TruSeq Adapter, Index 1 (97% over 37bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA267300.13514280089294356No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT255800.12932857638763548TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC244850.12379242348910299No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA216760.10959054815396349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)