FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19779078
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG978060.4944922104053586Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT959710.48521473043384533Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG927030.46869222114397857Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG791990.4004180579094739Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG755530.3819844383039493Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG663770.33559198259898665Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA653930.3306170287614013Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG621630.31428664167257947Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT601980.30435190153959657Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA500280.2529339335230894Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT460500.23282177258211936No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG460420.232781325802952Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG356100.1800387257687138Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT333760.16874396268622835No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA310130.1567969952896692Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG304370.15388482718961927Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATG280650.14189235716649684Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA264780.13386872734917168Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG263240.1330901268502No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC237070.11985897421507717No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG232460.117528228565558Illumina Single End PCR Primer 1 (100% over 22bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC218120.11027814339980863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)