FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15030803
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG864150.5749193838812204Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC834000.5548605753132417Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC815790.5427454541184527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC686240.4565557808188957Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC677550.4507743199082577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA617640.4109161699478065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG610140.4059264165726874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT598100.3979161991544963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT586620.39027854998831396TruSeq Adapter, Index 18 (97% over 38bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC518720.3451046494322359Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC478940.3186389975306043No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT430330.2862987426553325TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG403050.26814934637889937Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG346800.2307261960655063No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC327220.2176996132541954Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA297560.19796680190672447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT290590.19332965777011382Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG246790.16418949805941838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA244600.1627324900738836No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCA244420.16261273599288076TruSeq Adapter, Index 1 (97% over 37bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT237910.1582816300632774No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT230250.15318542861615578TruSeq Adapter, Index 1 (97% over 35bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA207840.13827604553129996No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC198460.13203552731015103No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA169320.11264867219668835No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCA156000.10378687020247687TruSeq Adapter, Index 1 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153720.10226998517644068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)