FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53613550
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT869060.16209708180114915No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC804830.15011690141764536Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC799050.14903881574713856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT713780.13313425430698023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)