FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9648170
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1183131.2262739980742463No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1137011.1784721869535881No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA695780.7211523014208912No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC519850.538806841090072No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC349830.36258689471682193No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC345770.35837884282718896No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCG249990.2591061310072273No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT240950.24973647852390662No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC240700.24947736202823953No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC234040.24257449858366922No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTC233090.24158985590013443No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC211860.21958568308808823No Hit
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC199680.20696152741918936No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA198130.2053550051460536No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC195060.20217305457926218No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC192820.19985137077808537No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA192340.19935386710640463No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG177850.1843354750175422No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG168260.174395766243754No Hit
CCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCG165360.17139001489401617No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG163010.16895431983474588No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA151990.15753246470574211No Hit
GGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCCGACC151860.15739772412799527No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC149700.1551589576054319No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG148820.15424686754068387No Hit
CTCCCGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAG145590.15089908241666555No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC143860.14910599626664953No Hit
GCGGGGCACGCGCCCTCCCGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCC141490.14664957188772587No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC138380.14342616268162772No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC137200.14220313282207922No Hit
CCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCGCGC131660.1364611112780973No Hit
CGCGCCCTCCCGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGGCGG130070.13481313036565482No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG127760.13241889394569126No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG125870.13045997323844832No Hit
CGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGA123780.12829375933467174No Hit
GTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCCGACCCGC121350.125775146996788No Hit
CCCGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGGC118410.12272793700774344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCTCGTAT116000.12023005398951304TruSeq Adapter, Index 4 (97% over 37bp)
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT115880.12010567807159286No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG115260.11946306916233856No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCC111610.11567996832559957No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA110640.11467459632241139No Hit
CGCCCTCCCGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGGCGGGG105920.10978247688421743No Hit
CCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGG105850.10970992426543064No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA104950.10877710488102926No Hit
CGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGC102130.10585427080990488No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG101330.1050250980237703No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG100490.10415446659832901No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG100460.10412337261884896No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG100180.10383316214370186No Hit
GGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCCGA100070.10371915088560836No Hit
CGCGGCGGGGCGCGTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGGCGG97610.10116944456824455No Hit
GTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGC96570.1000915199462696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)