FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27359545
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5212021.905009750710401No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2768411.0118625876270968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT812960.2971394443876899TruSeq Adapter, Index 23 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT747310.2731441623024067No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG454820.1662381446767481Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC454100.16597498240559191Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC418380.15291920973100978Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC388600.14203452579346623Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCGCGTAT376570.13763752284623154TruSeq Adapter, Index 23 (97% over 37bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA360000.1315811355780953Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG349140.12761177132148946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC343980.1257257750448701Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT322800.11798441823502546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG304580.11132495076215633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)