FastQCFastQC Report
Wed 11 May 2022
EGAF00005408151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27359545
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4056571.4826891309778727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3117671.1395182193271123No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT474350.17337642128185976Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG467710.17094948033675267Illumina Single End PCR Primer 1 (100% over 30bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG449830.16441428393637395No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG435090.15902676744075972Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG403160.1473562517212914Illumina Single End PCR Primer 1 (100% over 32bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA359430.13137279878009667No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG346260.1265591222368647Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG344680.1259816272529386Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG318660.11647123517587739Illumina Single End PCR Primer 1 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG318220.1163104137879486No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA317620.11609111189531843Illumina Single End PCR Primer 1 (100% over 26bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT309570.11314881150253048No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT275960.10086425048369775Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)