FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7305572
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1104741.5121882311200274No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592000.8103403812870505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT290640.3978333250291695TruSeq Adapter, Index 23 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT206950.2832769288975593No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA166070.22731964040598054No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC137980.18886953684119465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG137240.1878566113645858Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCGCGTAT130870.17913723935648024TruSeq Adapter, Index 23 (97% over 37bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC126840.1736209019635971Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC117240.16048024713191522Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA111030.15197988603767096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC104970.14368484767517176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG103450.14160424399348878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT99430.13610159478272202Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG89900.12305675722585445No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC81460.11150393151966745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)