FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7305572
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT838211.1473571131733422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649920.8896223321048646No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG214930.29420009822639487No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT139890.1914839796254147Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG134820.1845440712924327Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG126750.17349770832455008Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG116670.1597000207512841Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114840.1571950834239947No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG101080.13836014483191733Illumina Single End PCR Primer 1 (100% over 28bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA96500.13209095742263574No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG95800.13113278467449227Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA91280.12494572635790872Illumina Single End PCR Primer 1 (100% over 26bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT83220.11391305157214247No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT82690.1131875779199767Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG82580.11303700791669702Illumina Single End PCR Primer 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)