FastQCFastQC Report
Thu 12 May 2022
EGAF00005408158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31824437
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC887350.27882661364912753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC867100.2724635788529425Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC604730.19002064357022247Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG554160.17413033889649013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG499690.15701456085460364TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC435090.13671569429492186Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT364860.11464774694993032No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC344060.10811188898644146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA333490.10479054193480311TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)