FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31824437
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG861640.27074791613752663Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG839120.26367159299628773Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG590860.18566235751476137Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG532630.16736509745639805Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC489790.15390374384313538Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG424600.1334194851585277Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCGG328240.10314086624690329Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT326700.10265696137845266No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG325780.1023678753531445Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)