FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10899250
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352350.3232791247104158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC339050.3110764502144643Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC239680.2199050393375691Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG215440.19766497694795512Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG198890.182480445902241TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC171320.15718512741702412Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC136200.12496272679312798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT128370.11777874624400761No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA128020.11745762323095625TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG112810.1035025345780673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)