FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51990351
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1261740.24268734019510657No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG793610.15264563226357136No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC686780.1320975886467856No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC679940.130781959906368No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC654070.1258060365855195No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT646330.12431729880031009No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA609030.1171428906106058No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC605610.11648507624039699Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA596670.11476552639546518No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT548270.1054561066533288No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC532030.10233245011175246Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT527900.10153807193954123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)