FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19025392
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1531110.8047718543723041Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1508470.7928719681570818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1481800.7788538601464822Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1351150.7101824761350515Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1130750.5943372940752022Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC996510.5237789581418348Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT956780.5028963397968357TruSeq Adapter, Index 1 (100% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT903360.47481807470773796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA805330.42329219813184404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG762420.400738129337887Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG659000.34637919681234425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAA613350.32238494744287005TruSeq Adapter, Index 1 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC570410.2998151102484511Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT475020.24967685291320146TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT469100.24656522188872637Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC444030.23338809523609289No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA428030.22497828165643055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACG367770.19330482126202708TruSeq Adapter, Index 1 (97% over 39bp)
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAA339480.17843521962648654TruSeq Adapter, Index 1 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG298060.15666431472213554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG259430.13635987106073819No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259390.13633884652678904No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT251340.1321076590695214TruSeq Adapter, Index 1 (97% over 39bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA217760.114457562819205No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT209840.1102947050972721No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC199830.10503331547649583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)