FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29316565
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2803560.9563057609239008Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2775290.9466627485177749Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2716980.9267729694798829Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2462850.8400881890494334Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC2059560.7025243237057275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1838620.6271607877662339Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1742970.5945341822959136TruSeq Adapter, Index 1 (100% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1636970.5583771495739696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1432520.4886384199513142Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1382870.47170260226598854Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1174540.40064038880407715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAA1123360.38318268187285925TruSeq Adapter, Index 1 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1032570.3522138422424319Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT889160.3032961058022998TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT838870.2861419815043133Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC775050.26437271897304476No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA764370.26072972737426775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACG678150.23131973339987136TruSeq Adapter, Index 1 (97% over 39bp)
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAA628960.21454082359239562TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT563970.19237246928485655TruSeq Adapter, Index 1 (97% over 39bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG516190.1760745162333991Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG437740.1493149009783377No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC343170.11705668791688248No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT311520.10626074371264163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)