FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29316565
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT2785110.9500123906057889Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2700730.9212300281427922Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2686770.9164682151541287Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2429930.8288590426606938Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1969130.6716782815449218Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1817600.6199907799566559Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA1730510.5902840254306737Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1512330.5158619367582798Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1340520.457256844381325Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1270760.43346142360129847Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAAC1102310.3760024409408128Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG972150.3316043335909238Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG972140.33160092255010093Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA879180.2998918870611206Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA743130.25348467666658764Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAACCT650700.22195642634121698Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG629150.2146056333680293Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAAC608360.2075140794973763Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT605180.20642936851571797No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG378010.12894075414360448Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT311150.10613453520219712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)