FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79947576
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG4609160.5765227953878177Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC3904920.4884350715023555Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC3701190.46295212252589124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT2783750.34819692344393277Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2383510.29813411728705824Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2361780.29541608616126147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2211340.27659875516425914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAACATCTCGTAT2065620.2583718110477796TruSeq Adapter, Index 11 (97% over 37bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1936290.24219496035752228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1665320.20830149997293226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1372010.1716137084631559TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1194150.14936662995260794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1128910.1412062824769071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAACATCGCGTAT1107200.13849075299043465TruSeq Adapter, Index 11 (97% over 37bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1105940.13833314971300692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC986870.12343963999608942TruSeq Adapter, Index 5 (97% over 35bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT982730.12292180065596986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA868350.10861492536058881No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA866060.10832848765796226No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT819970.10256345983523003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT803330.10048209591745472TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)