FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79947576
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4839990.6053954656486396Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG4118860.515195107353849Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG3864290.4833529912151433Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA2850910.35659742829476154Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2516740.31479878764554414Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2501850.3129363171686406Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2327920.2911808107853076Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1960230.24518942262864857Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1751630.2190973244767296Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGC1443380.1805408083917391Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1234340.15439367417468666Illumina Single End PCR Primer 1 (100% over 28bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1088570.13616047595989653No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG1083330.13550504645694325Illumina Single End PCR Primer 1 (100% over 22bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG1072270.13412163991063344No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGC1031860.12906707765598796Illumina Single End PCR Primer 1 (97% over 35bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC852990.10669366635956543No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTC840550.10513764669988243Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA817250.10222323688713214Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)