FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39963896
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT1406750.35200521991149214TruSeq Adapter, Index 11 (97% over 36bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1265450.3166483067616831Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1128860.28246995738353436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1073410.26859493378723637Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1069730.26767410264504743Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1046790.26193392155759787Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1045170.2615285556743516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC822680.20585580544999915No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC623430.1559983040692529Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT588030.14714030884276147No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC582040.1456414559781659Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG574780.1438248162791736No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC535220.1339258815006425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTAT521450.1304802714930496TruSeq Adapter, Index 11 (97% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG434870.10881571706622398Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)