FastQCFastQC Report
Thu 12 May 2022
EGAF00005408185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39963896
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1186730.2969505275461632Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1031940.2582180676278409Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1029270.2575499645980462Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG937280.23453168830186127Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA932370.23330307935942982Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG857300.21451862451048317Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT630020.1576472924461619No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG592110.14816123032649267Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG550350.13771179866947908Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG504280.1261838935823474Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG449460.11246651227397848No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT425140.10638101950820811No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG399810.10004279863004346Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)