FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311203
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT60240.4594254284042974TruSeq Adapter, Index 11 (97% over 36bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG52930.4036750983638689Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC45980.3506703386127091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC44050.33595103122857406Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT42680.32550261096107924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG42420.32351969908549627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA42090.3210029263203333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC31150.23756809586311195No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26800.2043924548677817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTAT24350.18570732373248078TruSeq Adapter, Index 11 (97% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC24020.1831905509673178Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC22550.17197947228613722Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG21380.1630563688460139No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT20140.15359940451631057No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG16790.12805034765783788Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC14920.1137886353219143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT13930.10623831702642535No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT13800.10524686108863386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA13540.10326394921305092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)