FastQCFastQC Report
Fri 27 May 2022
EGAF00005408191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61699784
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1120270.18156789657480812No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA899680.14581574548137802No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT888450.14399564186480782No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG864780.14015932373442347Illumina Single End PCR Primer 1 (96% over 33bp)
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT832460.1349210558014271No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG739670.11988210525988227Illumina Single End PCR Primer 1 (100% over 32bp)
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG707360.11464545807810284No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG701940.11376701091854713Illumina Single End PCR Primer 1 (100% over 32bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA685200.1110538733814692No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG641430.10395984530513105Illumina Single End PCR Primer 1 (100% over 30bp)
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA619850.1004622641790772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)