FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8326049
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC262070.3147591372570592No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG214440.2575531323440446No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA160130.19232411435483984No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG152280.1828958729404547No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC133080.15983571559571652Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT130820.157121342908263No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC129600.15565606207698274No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC127820.15351819332314762No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG127720.1533980883369771No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA126610.15206492299048446No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC114450.13746015667215028Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA112130.1346737209929944No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC107710.12936508060425778No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC106850.12833217772319142Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA104320.12529352157307747No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG103440.12423659769477695No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC101310.12167836148934508No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT100450.12064545860827867No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG98990.11889192581018919Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT95110.11423185234677335No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC92860.11152949015793687No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC90060.10816655054516253No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC87190.10471953744206886Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT84200.10112839835557058TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)