FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005408198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29777060
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT11952594.014026233617423TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT7144582.3993570889805778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCGCGTAT6828242.293120946124298TruSeq Adapter, Index 9 (97% over 36bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2888310.9699782315648355Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2835240.9521557870387473Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC2157990.724715603219391Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1924470.646292817356717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1908850.6410471685250324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1864370.6261094950273801Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1780610.5979804587827005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA1756860.5900045202582122No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1342660.45090415239113596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1288380.432675354786537Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC1260580.42333930885050436No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA1220920.4100203310870852No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG1120650.37634675820917174No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA947780.31829200062061197TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG924600.31050748462071137Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA870250.2922551789867771TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG763310.25634162674219685No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC724930.24345251008662372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG696170.2337940683197065No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT662080.22234565803339887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA657640.2208545773155577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG622980.20921474450466232TruSeq Adapter, Index 9 (97% over 36bp)
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG588460.19762192775243764TruSeq Adapter, Index 9 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC524320.1760818563014616No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG515920.1732608927812215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC473810.15911913399106561No Hit
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA408820.13729360789816053No Hit
ATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCA384050.12897512380335735No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGC382030.1282967492425377TruSeq Adapter, Index 9 (97% over 36bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA368930.1238973894669252No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC368210.12365559259376177No Hit
GTGGGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315990.10611860270960263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)