FastQCFastQC Report
Thu 12 May 2022
EGAF00005408199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29777060
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT4210451.4139911730708137No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2943790.9886100239580402Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2900100.9739376553628868Illumina Single End PCR Primer 1 (100% over 32bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG2888260.9699614401153103No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG2186460.7342766545790619Illumina Single End PCR Primer 1 (100% over 28bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA2048220.6878516549316823No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1943510.6526870013359277Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1893560.6359123432602144Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1850570.6214750549584143Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1818880.6108326342493181No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG1697270.5699924707140329Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1367880.45937375953166626Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1313480.4411046624482068Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT1159180.38928624921332056No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCT962120.3231077883444504Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG936260.31442325064999704Illumina Single End PCR Primer 1 (100% over 30bp)
TCCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGT889930.29886429351991095No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCT875200.2939175324897757Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG727230.2442249167647847Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT665720.2235680755588362No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA642350.21571975205073973Illumina Single End PCR Primer 1 (100% over 26bp)
CAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATT639200.21466189073064973No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCG630390.21170323732430268Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG621660.208771450237196Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCG588080.19749431273604579Illumina Single End PCR Primer 1 (96% over 33bp)
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC579940.19476066475333698No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG466270.15658698340265964Illumina Single End PCR Primer 1 (100% over 22bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG416460.13985934138561698No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCGCA386980.12995910274553632Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGTA335980.11283182422979302No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT316810.10639398248181654No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC307710.10333793866822312No Hit
ATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCA304660.10231366024718357No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT303500.10192409861819803No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298060.10009718890985207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)