FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624238
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT1153524.395637895648184TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCGCGTAT672532.562762981101562TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT626292.386559450781522No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA334951.2763705121258055No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG271771.035614909928139Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC267711.020143752205402Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC201620.7682992167631136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC181670.6922771486427679Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA176360.6720427034438188Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT171930.6551616126281229Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG163130.6216280687955894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC127090.484292964281441Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG121330.4623437355910554No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC119560.455598920524739Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC111830.42614275077184316No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA105340.4014117621953497No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA89350.3404797887996439TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG85740.32672341456834325Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA82890.3158631191225796TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG67300.25645539771926174No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC66640.2539403819318217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT61670.23500155092640224Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG60890.23202925954124587No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA59830.22798999176141796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA59670.22738029096446283No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG57580.21941607430423613TruSeq Adapter, Index 9 (97% over 36bp)
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG56990.21716780261546398TruSeq Adapter, Index 9 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC45170.17212615624040198No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG45090.1718213058419244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC44110.16808688846057407No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGC36360.1385545061080588TruSeq Adapter, Index 9 (97% over 36bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC33520.1277323169621048No Hit
ATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCA33250.126703446867243No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA31550.12022537589959448No Hit
GTGGGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27030.10300132838561138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)