FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624238
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG435491.6594912504125006No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT350581.3359306587283621No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG268901.0246784018827562Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG266251.0145802324331863Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240280.9156181718274028No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG196960.7505416810517949Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT179160.6827124673905339Illumina Single End PCR Primer 1 (96% over 33bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA176240.6715854278461023No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA163330.6223901947917834Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG163320.6223520884919737Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG142370.5425193903906581Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT126630.48254007449019487Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG117810.44893031805804195Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT94130.3586946001086792No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCT89540.3412038084960282Illumina Single End PCR Primer 1 (96% over 33bp)
TCCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGT86080.32801902876187294No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG84270.3211217884963178Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCT81450.3103758119499832Illumina Single End PCR Primer 1 (96% over 33bp)
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC70360.2681159254610291No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG64710.2465858660685502Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCG57490.21907311760594886Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA57380.2186539483080422Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCG56300.21453846792859488Illumina Single End PCR Primer 1 (96% over 33bp)
CAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATT55470.21137564504439002No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG52790.2011631566953912Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT52740.2009726251963427No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCGCA36470.13897367540596547Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG35870.13668729741738364Illumina Single End PCR Primer 1 (100% over 22bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG34530.13158105324288422No Hit
CCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGTA30080.11462374982756898No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC26800.10212488348998834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)