FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348828
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC136933.9254302980265345Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC105933.036740169940486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA59891.716891992615272Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG58721.6833511071358949Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC54721.5686814131893083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT54111.5511942848624538Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA46441.3313151467198734TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG42931.2306924902817433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC30940.8869700826768493No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG29260.8388088112192829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC28440.8153015239602326Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG26940.7723003887302624Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA26790.7680002752072655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA24750.7095187312945062TruSeq Adapter, Index 7 (97% over 37bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT23650.6779845654591948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG23070.6613574598369397No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC22770.6527572327909457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA20120.5767885605513319No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC20050.5747818409072667No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT17790.509993463827445TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG17740.5085600926531127No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC16550.47444585870400313Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCG15350.440044950520027No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACC15040.43115804923916656TruSeq Adapter, Index 7 (97% over 37bp)
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGG13890.39819051222952284No Hit
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC13710.3930303760019264No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG13480.3864368685999977No Hit
GGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGG13040.3738232022658731No Hit
CTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGGTCGG11290.32365521116424145No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA10360.29699450732166No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA9930.2846675152224019No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG9660.27692731088100725No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTAT8620.24711319045489466TruSeq Adapter, Index 7 (97% over 37bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA8380.24023300881809945No Hit
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC7900.22647264554450905No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC7640.21901911543798092No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC7250.20783882027818867No Hit
CCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCGG7150.204972077929524No Hit
CCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGC7040.20181866134599286No Hit
CGGTGGTCGCCGTATCATAGATCGGAAGAGCACACGTCTGAACTCCAGTC7030.2015319871111264Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCG6850.19637185088352999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6660.19092504042106712No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA6500.18633825266320364TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGT6320.18117811643560722TruSeq Adapter, Index 7 (97% over 37bp)
GGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGAC6310.18089144220074077No Hit
GCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGC6020.17257788938961321No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT5980.17143119245014735No Hit
GGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGAT5810.16655773045741742No Hit
CCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGG5810.16655773045741742No Hit
CGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCC5450.1562374580022246No Hit
GGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCG5420.15537743529762518No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA5360.1536573898884264No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG5360.1536573898884264No Hit
CGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCG5340.15308404141869347No Hit
CCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCGGC5020.14391046590296652No Hit
CGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAG5000.14333711743323357No Hit
GCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGC4820.13817698120563718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.1298634283945096No Hit
GGCGGCGCGGAGCGGCGCGGCGGAGGCGACGGGAATCCGGCCGGCCCCGA4520.12957675415964315No Hit
CCCGGCGCGGCGCGGCGCGGCCGCGAGCCGGAGGCGGGCGCGCGACGGCG4490.12871673145504375No Hit
GAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATC4420.12671001181097846No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC4300.12326992099258088No Hit
GGGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGG4280.12269657252284792No Hit
CCCGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACG4250.12183654981824854No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA4200.1204031786439162TruSeq Adapter, Index 7 (97% over 37bp)
CGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGC4130.11839645899985093No Hit
CGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCG4120.11810978476498447No Hit
CGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCC4110.117823110530118No Hit
GGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACG4030.11552971665118626No Hit
CCGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGA4030.11552971665118626No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG4010.11495636818145331No Hit
GGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGG4000.11466969394658685No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA3970.11380967124198745No Hit
CCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGGTCG3960.113522997007121No Hit
GCCGGACGCCGGGCCGCCACCGGGGGCGGGCGGCGAACCGCGGCGACCGG3940.11294964853738806No Hit
GGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACC3840.11008290618872338No Hit
AGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCC3830.1097962319538569No Hit
GTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCGT3740.1072161638400587No Hit
CTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCGTGACCAC3610.10348939878679464No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT3590.1029160503170617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGA3530.1011960049078629No Hit
CCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCC3530.1011960049078629No Hit
CGGCCCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCG3510.10062265643812998No Hit
GGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCG3510.10062265643812998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)