FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348828
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG139483.998532227917484Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC107353.0774479112915247Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG58421.6747508800899011Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG54201.5537743529762518Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG53241.5262536264290711Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA50021.4339445228020686Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAA47871.3723095623057782Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG35261.0108133521391631Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG29250.8385221369844164Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG29010.8316419553476211Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG25060.7184056325753666Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATC24870.7129588221129037Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG24360.6983384361347139Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT23980.6874448152097882No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG22810.6539039297304116Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA22580.6473104223284829Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC18110.519167039343172No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC16940.4856261538637953Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT16440.471292442120472No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCGA15070.43201807194376596Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT12510.35862946781795035No Hit
GGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAG12350.35404268006008693No Hit
GGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAG11190.32078846881557677No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT10790.3093214994209181No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG10640.30502138589792105No Hit
GGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGG9740.279220704759939No Hit
GGGGGACCGGCCGCGACTGCGGCGGCGGCGGTGGTGGGGGGAGCCGCGGG8900.25514006903115577No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC8740.25055328127329224No Hit
GGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGG8350.23937298611350008No Hit
GGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCG8220.235646221060236No Hit
GGGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCG7840.22475260013531023No Hit
CGACGGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTCGCG7740.22188585778664557No Hit
CCGACGGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTCGC7610.2181590927333815No Hit
GCCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGT7510.2152923503847168No Hit
GGGGGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGA7260.20812549451305512No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA7120.2041120552249246No Hit
GTCCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGG7110.20382538099005815No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC7070.20267868405059228No Hit
GGGGGAGAAGGGTCGGGGCGGCAGGGGCCGGCGGCGGCCGCCGCGGGGCC7020.20124531287625996No Hit
GGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGA6890.19751854782299585No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC6870.1969451993532629No Hit
CCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGGGC6750.19350510853486533No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAA6750.19350510853486533Illumina Single End PCR Primer 1 (96% over 33bp)
GAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCG6460.18519155572373777No Hit
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCGACG6440.18461820725400485Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.17458460903367848No Hit
GGGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCC5900.1691377985712156No Hit
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC5830.16713107892715034No Hit
CGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCG5770.16541103351795153No Hit
ATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5740.16455101081335213Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGGAGGAGGAAGGCGGGTCC5620.16111091999495453No Hit
CGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCC5490.15738415494169047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480.157097480706824No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.15537743529762518No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG5370.15394406412329284No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC5270.15107732177462818No Hit
GCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGC5260.1507906475397617No Hit
GGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTC5160.14792390519109702No Hit
GGGGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAG5160.14792390519109702No Hit
CCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTG5140.1473505567213641No Hit
GTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTGG4960.1421904204937677No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC4740.1358835873267054No Hit
GCCCCGACGGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGT4700.13473689038723957No Hit
GCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGCCGGCGGCG4600.13187014803857489No Hit
GGCGGTTCTCGGCGGCGTCGCGGCGGGTCTGGGGGGTCTCGGTGCCCTCC4590.1315834738037084No Hit
GCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTG4530.1298634283945096No Hit
CGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGC4490.12871673145504375No Hit
GGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGC4430.12699668604584494No Hit
GGAGAAGGGTCGGGGCGGCAGGGGCCGGCGGCGGCCGCCGCGGGGCCCCG4400.12613666334124554No Hit
GGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCG4340.12441661793204674No Hit
GGGAGAAGGGTCGGGGCGGCAGGGGCCGGCGGCGGCCGCCGCGGGGCCCC4340.12441661793204674No Hit
GGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGC4330.12412994369718026No Hit
GGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGG4220.12097652711364913No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATC4190.12011650440904972Illumina Single End PCR Primer 1 (96% over 33bp)
GGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTCGCGGCGG4110.117823110530118No Hit
GGGGTCTCGGTGCCCTCCTCCCCGCCGGGGCCCGTCGTCCGGCCCCGCCG4040.11581639088605272No Hit
CCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCGG4000.11466969394658685No Hit
GGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCG3890.11151627736305572No Hit
CGCGAGGCGGTTCTCGGTGGGGGCCGAGGGCCGTCCGGCGTCCCAGGCGG3880.11122960312818925No Hit
GGGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGGAGGAGGAAGGCGGGTC3780.10836286077952459No Hit
GGGGGGAGAAGGGTCGGGGCGGCAGGGGCCGGCGGCGGCCGCCGCGGGGC3640.10434942149139405No Hit
GGCGTGTTGCGTGCGGTGTGGTGGTGGGGGGAGGAGGAAGGCGGGTCCGG3640.10434942149139405No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG3630.10406274725652756No Hit
CCCCGACGGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTC3590.1029160503170617No Hit
GTGGGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGC3530.1011960049078629No Hit
CCCGACGGCGTGGGTGTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTCG3520.10090933067299643No Hit
GTCGGCGGGCGCGGGGGCGGTTCTCGGCGGCGTCGCGGCGGGTCTGGGGG3520.10090933067299643No Hit
GCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCG3510.10062265643812998No Hit
GGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCG3490.10004930796839702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)