FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8067577
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3360394.165302667703079Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2604783.2287017527071638Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1436431.7804974157668403Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1433301.7766176883096376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1335721.655664395889869Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1287831.596303326265123Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA1130361.4011146097520977TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1008341.24986721539813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG750890.9307503355716346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC722150.8951262566195526No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC697450.8645098770052024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA644700.7991246938207097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG641590.7952697569542875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA636450.7888985751236091TruSeq Adapter, Index 7 (97% over 37bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC580830.7199559421620643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT573920.7113907930472805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG525120.6509017515420057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT496090.6149182090236015TruSeq Adapter, Index 7 (97% over 37bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC461490.5720304869727305No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA439410.5446616747506717No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC430350.5334315371269466Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCG427200.5295270190789626No Hit
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC424980.5267752635022882No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG384740.47689659485121744No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACC354580.43951238395369513TruSeq Adapter, Index 7 (97% over 37bp)
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG346610.42963333352752625No Hit
CTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGGTCGG317960.3941208122339582No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG297960.3693302214531079No Hit
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGG291810.36170711478799644No Hit
GGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGG283500.35140662431855313No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA277730.3442545388782778No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT265430.32900832554805487No Hit
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC252500.3129812086082352No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC236090.2926405288725475No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTAT233700.2896780532742359TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA210020.2603259937897091No Hit
CGGTGGTCGCCGTATCATAGATCGGAAGAGCACACGTCTGAACTCCAGTC185580.23003189185551004Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA175050.21697964580939233TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA174640.2164714386983849No Hit
CCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCGG169340.20990193214145955No Hit
GCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCG166720.20665436474916818No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC161070.19965102285357797No Hit
CCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGC159650.19789089090813758No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA157560.19530027417153872No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGT148050.1835123482552444TruSeq Adapter, Index 7 (97% over 37bp)
CCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGG137640.17060884575381186No Hit
GGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGAC136650.16938171151015974No Hit
GCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGC135400.1678322995863566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134060.1661713300040396No Hit
CCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCGGC127440.15796564445557817No Hit
CGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCC120240.14904103177447206No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG119320.14790066459855294No Hit
GGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGAT119050.14756599162301146No Hit
GGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCG118740.1471817374659083No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA117310.1454092102250775No Hit
CGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCG114660.14212445694661482No Hit
CGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAG113890.1411700192015521No Hit
CCCGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACG109070.13519548682336716No Hit
CCCGGCGCGGCGCGGCGCGGCCGCGAGCCGGAGGCGGGCGCGCGACGGCG108130.1340303290566672No Hit
CCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGGTCG107420.133150263083947No Hit
GGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGG106330.13179917588639067No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA105410.13065880871047156TruSeq Adapter, Index 7 (97% over 37bp)
GGGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGG105330.13055964634734815No Hit
CGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCC104130.12907221090049714No Hit
GGCGGCGCGGAGCGGCGCGGCGGAGGCGACGGGAATCCGGCCGGCCCCGA104030.12894825794659287No Hit
GCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGC101420.1257130858496919No Hit
GGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCC98690.12232917020810585No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG98470.1220564737095165No Hit
GAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATC97620.12100287360133036No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT93590.11600756955898901Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGC92840.11507792240470714No Hit
CGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCGGTCGGCC92610.11479283061072736No Hit
CCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCC91190.11303269866528699No Hit
GCCGGACGCCGGGCCGCCACCGGGGGCGGGCGGCGAACCGCGGCGACCGG89570.11102466081203811No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA87690.1086943452786382No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC86920.10773990753357544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86630.10738044396725313No Hit
CTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCGTGACCAC86440.10714493335483503No Hit
GTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCGT85700.10622768149594357No Hit
GGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGC85350.1057938461572787No Hit
CGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCG83060.10295532351287133No Hit
CCGGGAAGGCGCGAGAAAGGCGGCCGGCGGGGAAGGGGACGCCACGGGGA82440.10218681519866499No Hit
GGCGGCTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACG82170.10185214222312348No Hit
CGGCCCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCG81800.10139351629367777No Hit
AGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCC81230.10068698445642353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)