FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54727683
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1039660.1899696722041019No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC977120.17854218312147438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC842130.153876421188889Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC602040.11000648428693756No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG600480.10972143658996125No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA568180.10381948747949005No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC556600.1017035564980889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)