FastQCFastQC Report
Wed 11 May 2022
EGAF00005408230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27998505
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2349340.8390948016688748Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1892850.6760539535950223Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG962810.34387907497203873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA921300.32905328338066625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC873900.3121238080390364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA846560.3023590009538009TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT839900.2999803025197238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG650650.23238740782766795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG610720.21812593208101644Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC589950.21070767885642466Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA509590.18200614639960241Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG499010.17822737321153398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG472190.1686482903283586TruSeq Adapter, Index 7 (97% over 36bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA466760.16670890106453898No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC465900.16640174180728579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT443850.15852632131608455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC427900.15282958857981882No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC368850.13173917678818922Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC332840.11887777579552908No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG309110.11040232326690301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)