FastQCFastQC Report
Wed 11 May 2022
EGAF00005408231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27998505
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2313940.8264512694517082Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1880860.6717715820898295Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG918560.3280746597005804Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTT859340.3069235303813543Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG807360.28835825341388766Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG768150.27435393425470395Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA732120.2614853900235031Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG592420.21158986881621Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG583990.2085789937712746Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG493260.176173692131062Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAA482030.17216276369041847Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG457550.16341943971651343Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG443320.1583370254947541Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG418050.14931154359848858Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA407270.1454613380250124Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG365150.13041767765814638Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG351440.12552098763844713No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT294300.10511275512746128No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC288240.10294835384960734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)