FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721065
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC540720.9451387110616642Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC434180.758914642640837Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG218860.38255115087837666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA204720.3578354729407899Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC197130.34456871229395225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA195080.34098546337089336TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT188780.329973527656127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG141990.24818805589518736Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG141300.24698198674547484Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC137940.24110895436426608Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA115900.20258465862562303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG111840.19548807783166247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG109800.19192230817164285TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC107090.1871854278879894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT101310.17708241385126722Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA98300.17182115567643436No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC93860.16406036288697995No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC85410.14929038561876154Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC72660.12700432524363908No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG64480.11270628807748208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCTCGTAT63020.11015431567374258TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)