FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31778604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2244610.7063274396823724Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2042450.642712310458949Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1408080.44309057754708164Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATC1385250.4359064985988686Illumina Single End PCR Primer 1 (97% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1158730.36462583441361995Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1154550.3633104839973461Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1114480.35070137127483636Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA952770.29981493208449306Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCTC943840.29700486528609Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG885260.2785710788302721Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG850670.2676863967970399Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA731780.230274432445176Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA685570.2157332021255559Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG626570.1971672512738445Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG618410.19459948586791287Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG547340.1722353820199276Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCTCGC538050.16931203145361579Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT383220.12059057093886187No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT364110.11457709092570587No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATC333860.10505810765004027Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)